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A Whole Genome Knockout Collection for Vibrio natriegens

Project Advisor
Professor Buz Barstow- bmb35@cornell.edu

Project Area/Concentration
synthetic biology, and a background in one or more of molecular, micro or synthetic biology; physics; or chemistry

Project Description
Vibrio natriegens is a new genomic powerhouse for synthetic biology and directed evolution. However, it currently lacks the most useful tool for gene characterization for any organism: a whole genome knockout collection: a collection of single-gene knockout mutants for every non-essential in an organism’s genome. We will use Knockout Sudoku, a new technology developed by our team for making knockout collections at ultra-low-cost and high speed, to build a knockout collection for V. natriegens to learn how to fully leverage its potential.

Possible Courses

Course number Course title
AEP 4210 Mathematical Physics I
AEP 4220 Mathematical Physics II
BEE 3600 Molecular and Cellular Bioengineering
BME 6130 Engineering the Microbiome