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Sudoku Gene Assembly

Project Advisor
Professor Buz Barstow-

Project Area/Concentration
synthetic biology and the future of technology, background in electrical and computer engineering, mathematics, physics, chemistry or synthetic or molecular biology

Project Description
Biology could make the control of matter and energy as easy at the control of information is today. The most advanced artificial organisms of tomorrow could have completely synthetic de novo genomes. Whole genome synthesis is possible today, but it’s extremely high cost places it out reach for almost all synthetic biologists. This makes iterative design, and garage synthetic biology almost impossible. We developed Knockout Sudoku, a new technique for making whole genome knockout collections. Knockout Sudoku drops the cost of making a knockout collection from millions of dollars to only thousands, and the construction time from years to weeks. We aim to use the same approach to develop a new technology for rapid, ultra-low-cost whole genome assembly.

Possible Courses

Course number Course title
AEP 4210 Mathematical Physics I
AEP 4220 Mathematical Physics II
BEE 3600 Molecular and Cellular Bioengineering
BME 6130 Engineering the Microbiome