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Playing 8-dimensional Sudoku to Discover Genetic Network Maps

Project Title
Playing 8-dimensional Sudoku to Discover Genetic Network Maps

Project Advisor
Professor Buz Barstow -

Project Area/Concentration
systems and synthetic biology, background in systems, synthetic or molecular biology; mathematics; physics; or electrical or computer engineering

Project Description
Genomes could hold the keys to making it as easy to manipulate matter and energy as it is to manipulate information today. However, despite enormous advances in gene sequencing, we know very little about what most genes actually do, making exploiting them a tough challenge. Genetic network analysis has the potential to reveal the function of many more genes. However, the most useful tool for genetic network analysis, a double-gene knockout collection, requires a phenomenal amount of time and money to construct, and so far only exists for the baker’s yeast Saccharomyces cerevisiae. My lab has built Knockout Sudoku, a new technology for constructing that drops the cost of whole genome single-gene knockout collection construction from millions of dollars and years of work to only a few thousand dollars and a few weeks. We need help to upgrade Knockout Sudoku to build a rapid, generalizable low-cost method to democratize building double-gene knockout collections.

Possible Courses

Course number Course title
AEP 4210 Mathematical Physics I
AEP 4220 Mathematical Physics II
BEE 3600 Molecular and Cellular Bioengineering
BME 6130 Engineering the Microbiome